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Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells
Author(s) -
Jia Liwei,
Miao Chenglin,
Cao Yujing,
Duan En Kui
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.03.011
Subject(s) - wnt signaling pathway , hek 293 cells , wnt3a , apoptosis , cell growth , microbiology and biotechnology , wnt5a , carcinogenesis , cell culture , cancer research , signal transduction , biology , chemistry , biochemistry , genetics , gene
Wnt proteins and Wnt signalings have been implicated in a variety of development and cell processes, while aberrant activation of Wnt signaling is linked to a range of cancers in many tissues. In this study, we used the HEK293 cell line to investigate the effects of Wnt3a and Wnt5a on proliferation and apoptosis in a serum starvation culture. After Wnt3a and Wnt5a proteins were expressed, they both promoted the proliferation of HEK293 cells under serum starvation. After 48 h of serum starvation, both Wnt3a and Wnt5a inhibited serum starvation‐induced apoptosis of HEK293 cells and continued up to 96 h. We demonstrated that Wnt3a and Wnt5a can promote proliferation of HEK293 cells and inhibit serum starvation‐induced apoptosis, which implies that Wnt3a and Wnt5a can maintain the survival of HEK293 cells under stress, and also provide a novel insight into the role of Wnt3a and Wnt5a and their related signalings in carcinogenesis.