Hyperthermia in combination with oxidative stress induces autophagic cell death in HT‐29 colon cancer cells | Zendy

Chen Fei | Zendy; Wang ChiaChi | Zendy; Kim Eugene | Zendy; Harrison Lawrence E. | Zendy

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Hyperthermia in combination with oxidative stress induces autophagic cell death in HT‐29 colon cancer cells

Author(s) -

Chen Fei,

Wang ChiaChi,

Kim Eugene,

Harrison Lawrence E.

Publication year - 2008

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1016/j.cellbi.2008.02.010

Subject(s) - autophagy , programmed cell death , vacuole , autophagosome , apoptosis , microbiology and biotechnology , hyperthermia , cytoplasm , chemistry , organelle , oxidative stress , cell , viability assay , cancer research , biology , biochemistry , paleontology

The purpose of this study was to evaluate the mechanism of ROS‐induced hyperthermic cell death in a colon cancer cell line. HT‐29 colon cancer cells were exposed to heat (43 °C) in the presence of tert ‐butyl hydroperoxide ( t ‐BOOH). t ‐BOOH combined with hyperthermia significantly decreased cell viability as compared with t ‐BOOH or hyperthermia alone. This decrease in cell numbers was associated with retardation in the S phase transit and not through apoptosis. Cell death was noted to be accompanied by specific features characteristic of autophagy: the presence of cytoplasmic autophagic vacuoles; autophagosome membrane association of microtubule‐associated protein light chain 3; accumulation of acidic vesicular organelles; and increased incorporation of MDC in the autophagosome. Thermal sensitization through modulation of cellular ROS may represent a novel approach to increase the efficacy of hyperthermia as an anticancer modality.

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