Premium
The peripheral chromosome scaffold, a novel structural component of mitotic chromosomes
Author(s) -
Sheval Eugene V.,
Polyakov Vladimir Y.
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.01.290
Subject(s) - chromosome , scaffold , mitosis , chromosome segregation , biology , microbiology and biotechnology , computational biology , genetics , computer science , database , gene
Using an original high‐salt extraction protocol, we observed a novel chromosome substructure, referred to as the peripheral chromosome scaffold. This chromosome domain contained the perichromosomal layer proteins pKi‐67, B23/nucleophosmin and fibrillarin, but no DNA fragments (i.e., the loop domain bases were not associated with the peripheral scaffold). Modern models of chromosome organization do not predict the existence of a peripheral chromosome scaffold domain, and thus our observations have conceptual implications for understanding chromosome architecture.