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Effects of the herbicide isopropyl‐ N ‐phenyl carbamate on microtubules and MTOCs in lines of Nicotiana sylvestris resistant and sensitive to its action
Author(s) -
Yemets Alla,
Stelmakh Oksana,
Blume Yaroslav B.
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2008.01.012
Subject(s) - microtubule organizing center , microtubule , fragmentation (computing) , biology , cell division , spindle apparatus , microbiology and biotechnology , tubulin , mitosis , chemistry , botany , centrosome , cell cycle , cell , genetics , ecology
To clarify the mechanism of isopropyl‐ N ‐phenyl carbamate (IPC) action on higher plant cells the sensitivity of microtubules (cortical network and mitotic arrays) and microtubule organizing centers to IPC treatment (30 μM) in IPC‐resistant and sensitive Nicotiana sylvestris lines was studied. It was clearly demonstrated that IPC does not depolymerize plant MTs but causes the MTOC damage in cells, which results in MTOC fragmentation, splitting of the spindle poles and in abnormal division spindle formation. It was also found that IPC‐resistance of mutant N. sylvestris line correlates not with tubulin resistance to IPC action but possibly with resistance of one of the proteins involved in MTOC composition.