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Plant tubulin intronics
Author(s) -
Breviario Diego,
Gianì Silvia,
Ponzoni Elena,
Mastromauro Francesco,
Morello Laura
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.11.013
Subject(s) - intron , gene , biology , tubulin , genetics , coding region , sequence (biology) , computational biology , microtubule
Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5′UTR sequence of some rice β‐tubulin genes can sustain IME in rice transgenic plants and that degenerated oligonucleotide mixtures designed to amplify the first and the second intron present within the coding sequence of plant β‐tubulin genes can successfully detect ILPs among different bean varieties.