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Cloning and expression of the tubulin genes in barley
Author(s) -
Radchuk Volodymyr,
Sreenivasulu Nese,
Blume Yaroslav,
Weschke Winfriede
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.11.009
Subject(s) - gene , biology , tubulin , in silico , cloning (programming) , genetics , gene expression , microbiology and biotechnology , microtubule , computer science , programming language
Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for α‐, β‐, and γ‐tubulins were selected. Five genes for α‐tubulin, eight newly identified β‐tubulin sequences and one γ‐tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific.