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Aluminum ions inhibit phospholipase D in a microtubule‐dependent manner
Author(s) -
Pejchar Přemysl,
Pleskot Roman,
Schwarzerová Kateřina,
Martinec Jan,
Valentová Olga,
Novotná Zuzana
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.11.008
Subject(s) - phosphatidic acid , microtubule , phospholipase d , cytoskeleton , microbiology and biotechnology , phospholipase , phospholipase a2 , biology , phosphatidylinositol , biochemistry , phospholipid , chemistry , enzyme , signal transduction , cell , membrane
Aluminum is a highly cytotoxic metal to plants, but the molecular base and the primary target of Al toxicity are still unknown. The most important physiological consequence of Al toxicity is a cessation of root growth and changes in root morphology suggesting a role of the root cytoskeleton as a target structure. The important role of phospholipid degrading enzyme phospholipase D in regulation of cytoskeleton remodelling in both animal and plant organisms is now evident. Both the phospholipid pathway and the cytoskeleton are influenced by Al 3+ , but their relationship with Al stress remains to be explored. Therefore, we tested the possibility that Al stress could be sensed by plants through microtubules in close interaction with phospholipases. We have shown that Al 3+ reduced the formation of phosphatidic acid in vivo , inhibited activity of phosphatidylinositol‐4,5‐bisphosphate‐dependent phospholipase D in vitro and that the phosphatidic acid production is modified by microtubule dynamics.