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Identification of a novel small Arabidopsis protein interacting with gamma‐tubulin complex protein 3
Author(s) -
Janski Natacha,
Herzog Etienne,
Schmit AnneCatherine
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.11.006
Subject(s) - arabidopsis , microtubule , nucleation , arabidopsis thaliana , tubulin , microbiology and biotechnology , microtubule nucleation , cytoskeleton , biology , chemistry , cell cycle , biophysics , cell , mutant , biochemistry , gene , organic chemistry , centrosome
In higher plants, microtubules (MTs) show dynamic structural changes during cell cycle and development progression. A precise control of MT nucleation at dispersed sites is one way used to regulate the cytoskeletal organization. Some gamma‐tubulin complex proteins (GCPs) were previously identified in Arabidopsis thaliana ( At ). They are directly involved in the nucleation process. Nevertheless, no additional player which may anchor the nucleating complex or regulate the nucleation activity has been found in plant cells so far. Therefore, our aim was the identification of Arabidopsis proteins interacting with MT nucleating complexes and particularly with At GCP3. Performing a yeast two‐hybrid screen, we discovered a new protein which we called At GCP3 Interacting Protein 1 ( At GIP1). The possible role of this protein during the nucleation process is discussed.