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Oligomycin A and the IPLB‐LdFB insect cell line: Actin and mitochondrial responses
Author(s) -
Tettamanti Gianluca,
Malagoli Davide,
Ottaviani Enzo,
Eguileor Magda
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.10.011
Subject(s) - oligomycin , microbiology and biotechnology , organelle , reactive oxygen species , mitochondrion , autophagy , mitochondrial ros , actin , biology , programmed cell death , biochemistry , apoptosis , enzyme , atpase
Oligomycin A, an inhibitor of mitochondrial ATP synthase, provokes simultaneous and different responses in IPLB‐LdFB insect cell line. The oligomycin A treatment causes mitochondrial loss, increase in reactive oxygen species (ROS), destabilization/reorganization of the actin microfilaments and, finally, autophagic cell death. We speculate that oligomycin A affects the mitochondria and that the impairment of these organelles leads to the generation of ROS in quantities that exceed the antioxidant capacity of the cell. This in turn would lead to a feedback loop of increased mitochondrial impairment, amplification of ROS production and the removal of damaged organelles through autophagy.