Aqueous extract of the Chinese medicine, Danggui‐Shaoyao‐San, inhibits apoptosis in hydrogen peroxide‐induced PC12 cells by preventing cytochrome  c  release and inactivating of caspase cascade | Zendy

Qian Yunfei | Zendy; Wang Hua | Zendy; Yao Wenbing | Zendy; Gao Xiangdong | Zendy

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Aqueous extract of the Chinese medicine, Danggui‐Shaoyao‐San, inhibits apoptosis in hydrogen peroxide‐induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade

Author(s) -

Qian Yunfei,

Wang Hua,

Yao Wenbing,

Gao Xiangdong

Publication year - 2008

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1016/j.cellbi.2007.10.004

Subject(s) - hydrogen peroxide , chemistry , apoptosis , cytochrome c , caspase , aqueous solution , traditional medicine , aqueous extract , pharmacology , cascade , caspase 3 , biochemistry , medicine , programmed cell death , chromatography , organic chemistry

Danggui‐Shaoyao‐San (DSS), a traditional Chinese medicine used for centuries for the enhancement of women's health, has been shown to display therapeutic efficacy on senile dementia. In the present study, using a rat pheochromocytoma (PC12) cell line, the effect of DSS on hydrogen peroxide (H 2 O 2 ) induced apoptosis was studied. The apoptosis in H 2 O 2 ‐induced PC12 cells was accompanied by downregulation of Bcl‐2, upregulation of Bax, the release of mitochondrial cytochrome c into cytosol, and sequential activation of caspase‐9 and −3. DSS was able to suppress all these changes and eventually protected against H 2 O 2 ‐induced apoptosis. Taken together, these results suggest that treatment of PC12 cells with DSS can block H 2 O 2 ‐induced apoptosis by the regulation of Bcl‐2 family members, as well as suppression of cytochrome c release and caspase cascade activation.

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