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Establishment and characterisation of two novel breast cancer cell lines
Author(s) -
Bagadi Sarangadhara Appala Raju,
Kaur Jatinder,
Ralhan Ranju
Publication year - 2008
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.08.010
Subject(s) - cancer research , biology , cell culture , breast cancer , phenotype , suppressor , cancer , dna methylation , tumor suppressor gene , cell growth , cell , methylation , gene , gene expression , genetics , carcinogenesis
Two novel oestrogen receptor (ER) negative breast cancer cell lines, BCa‐11 (familial) and BCa‐15 (sporadic) were successfully established from primary tumours. Characterisation of these cell lines showed expression of epithelial specific antigen and cytokeratins confirming their epithelial lineage. Analysis of ultrastructure and anchorage independent growth confirmed the epithelial nature and transformed phenotype of these cells. Both cell lines showed loss of pRb, Dab2 and ERα and elevated levels of proliferation marker Ki67. In addition, BCa‐11 cells showed loss of HOXA5, tumour suppressor genes p16 INK4A and RARβ as well as overexpression of CyclinD1. Elevation of DNMT1 and DNMT3B transcript levels, promoter hypermethylation of RASSF1A, RAR β2, and HOXA5 further support their neoplastic origin. In conclusion, the two ERα negative breast cancer cell lines established herein have certain useful characteristics that may make them valuable for understanding the mechanism of oestrogen receptor negative breast tumours and testing new drugs.