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Interferon‐gamma and lipopolysaccharide stimulation increases matrix metalloproteinase‐9 expression and enhances invasion activity in ras / myc ‐transformed serum‐free mouse embryo cells
Author(s) -
Kidachi Yumi,
Yamaguchi Hideaki,
Umetsu Hironori,
Ryoyama Kazuo
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.06.018
Subject(s) - lipopolysaccharide , matrix metalloproteinase , interferon gamma , messenger rna , stimulation , nitric oxide synthase , matrix metalloproteinase 9 , interferon , nitric oxide , microbiology and biotechnology , biology , embryo , cell culture , chemistry , cancer research , immunology , cytokine , endocrinology , biochemistry , genetics , gene
Ras / myc ‐transformed serum‐free mouse embryo ( ras / myc SFME) cells were treated with interferon‐gamma (IFN‐γ, 100 units/ml) and/or lipopolysaccharide (LPS, 0.5 μg/ml) for 24 h to investigate the effects of these ligands on the expression of matrix metalloproteinase‐9 (MMP‐9) and tissue inhibitor of metalloproteinase‐1 (TIMP‐1). Aminoguanidine (AG, 1 mM; a nitric oxide synthase [NOS] inhibitor) was also added along with IFN‐γ and LPS to analyze a possible association of NO with invasiveness. Treatment of cells with IFN‐γ alone did not alter MMP‐9 mRNA expression or pro‐MMP‐9 production, but LPS alone and IFN‐γ + LPS co‐treatment enhanced them significantly. TIMP‐1 mRNA expression remained unchanged with or without treatment and the mRNA expression of MMP‐9 exceeded that of TIMP‐1 in LPS‐ or IFN‐γ + LPS‐treated cells. Co‐treatment of cells with IFN‐γ and LPS up‐regulated invasiveness and indicated a possible involvement of NO in the enhancement of invasiveness. These results suggest that ras / myc SFME cells respond to inflammatory and infectious conditions and that they may possibly modulate their characteristics as cancer cells due to their increase in MMP‐9 expression and invasion activity.