Premium
Epigallocatechin gallate‐induced apoptosis does not affect adipocyte conversion of preadipocytes
Author(s) -
Morikawa Keiko,
Ikeda Chiharu,
aka Mitsuko,
Pei Sumi,
Mochizuki Misa,
Mori Asuka,
Yamada Sonoko
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.05.013
Subject(s) - apoptosis , annexin , epigallocatechin gallate , catechin , chemistry , epicatechin gallate , adipocyte , gallate , biochemistry , microbiology and biotechnology , biology , adipose tissue , polyphenol , antioxidant , nuclear chemistry
In the present study, we examined the effect of epigallocatechin gallate (EGCG) on the growth and differentiation of human preadipocyte cells, AML‐I. EGCG exhibited cytotoxic activity on AML‐I cells, accompanied by the appearance of characteristics of apoptosis by Annexin V—FITC staining method. Among apoptosis‐related proteins examined, loss of NF‐κB and p‐Akt, and accumulation of Bad were displayed in EGCG‐treated cells by Western blot analysis. Among 6 structure‐related catechins including catechin (C), epicatechin (EC), catechin gallate (CG), epigallocatechin (EGC), epicatechin gallate (ECG) and EGCG, the catechins containing galloyl moiety exhibited apoptotic capacity. Interestingly, exposure of AML‐I to EGCG increased the amounts of cytoplasmic lipid droplets as well as the expression of fatty acid synthase and peroxisome proliferator activated receptor‐γ proteins. Our results suggest that EGCG induces growth arrest and apoptosis, but does not affect adipocyte conversion of preadipocytes.