Premium
Sensitivity to microcystins: A comparative study in human cell lines with and without multidrug resistance phenotype
Author(s) -
Votto Ana Paula Souza,
Re Viviane Plasse,
Yunes João Sarkis,
Rumjanek Vivian Mary,
Capella Márcia Alves Marques,
Neto Vivaldo Moura,
Freitas Marta Sampaio,
Geracitano Laura Alicia,
Monserrat José María,
Trindade Gilma Santos
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.05.010
Subject(s) - phenotype , biology , dna damage , k562 cells , catalase , hepatotoxin , multiple drug resistance , oxidative stress , antioxidant , cell culture , reactive oxygen species , lipid peroxidation , microbiology and biotechnology , cell , biochemistry , chemistry , gene , toxicity , dna , drug resistance , genetics , organic chemistry
Multidrug resistance (MDR) is an obstacle in cancer treatment. An understanding of how tumoral cells react to oxidants can help us elucidate the cellular mechanism involved in resistance. Microcystins are cyanobacteria hepatotoxins known to generate oxidative stress. The aim of this study was to compare the sensitivity to microcystins of human tumoral cell lines with (Lucena) and without (K562) MDR phenotype. Endpoints analyzed were effective microcystins concentration to 50% of exposed cells (EC 50 ), antioxidant enzyme activity, lipid peroxidation, DNA damage, reactive oxygen species (ROS) concentration, and tubulin content. Lucena were more resistant and showed lower DNA damage than K562 cells ( P < 0.05). Although microcystins did not alter catalase activity, a higher mean value was observed in Lucena than in K562 cells. Lucena cells also showed lower ROS concentration and higher tubulin content. The higher metabolism associated with the MDR phenotype should increase ROS concentration and make for an improved antioxidant defense against the toxic effects of microcystins.