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NF‐κB responds to mechanical strains in osteoblast‐like cells, and lighter strains create an NF‐κB response more readily
Author(s) -
Liu Jun,
Zou Ling,
Zheng Yi,
Zhao Zhihe,
Li Yu,
Yang Pu,
Luo Songjiao
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.04.005
Subject(s) - mechanotransduction , strain (injury) , nf κb , tensile strain , materials science , nfkb1 , blot , osteoblast , ultimate tensile strength , microbiology and biotechnology , chemistry , biophysics , signal transduction , composite material , biology , biochemistry , in vitro , anatomy , transcription factor , gene
Abstract This study was to examine the early responses of nuclear factor kappa B (NF‐κB) to mechanical strains in MG‐63. MG‐63 cells were subjected to cyclic uniaxial compressive or tensile strain, produced by a four‐point bending system, at 1000 μstrain or 4000 μstrain for 5 min, 15 min, 30 min and 1 h, respectively. Control cells received the same treatment with no mechanical stress loading. Expression of NF‐κB (p60) was measured by Western blotting. NF‐κB responded rapidly to mechanical stimuli in MG‐63 cells. NF‐κB was activated by cyclic uniaxial stretch at 1000 μstrain while it was restrained under a compressive strain environment at 1000 μstrain ( P < 0.001). The effects reversed for tension and compression at 4000 μstrain ( P < 0.001). Furthermore, strains at 1000 μstrain affected NF‐κB expression much easier than those at 4000 μstrain. This indicates that there may be different responding mechanisms or mechanotransduction pathways for different mechanical stimuli.