Premium
Caspase‐8 dependent osteosarcoma cell apoptosis induced by proteasome inhibitor MG132
Author(s) -
Yan XiaoBo,
Yang DiSheng,
Gao Xiang,
Feng Jie,
Shi ZhongLi,
Ye Zhaoming
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.03.037
Subject(s) - mg132 , apoptosis , proteasome inhibitor , cell cycle , cancer research , cancer cell , proteasome , microbiology and biotechnology , cell , chemistry , caspase 3 , biology , programmed cell death , cancer , biochemistry , genetics
Many researchers have reported that proteasome inhibitors could induce apoptosis in a variety of cancer cells, such as breast cancer cell, lung cancer cell, and lymphoma cell. However, the effect of proteasome inhibitors on osteocsarcoma cells and the mechanisms are seldom studied. In this study, we found proteasome inhibitor MG132 was an effective inducer of apoptosis in human osteosarcoma MG‐63 cells. On normal human diploid fibroblast cells, MG132 did not show any apoptosis‐inducing effects. Apoptotic changes such as DNA fragment and apoptotic body were observed in MG132‐treated cells and MG132 mostly caused MG‐63 cell arrest at G 2 –M‐phase by cell cycle analysis. Increased activation of caspase‐8, accumulation of p27 Kip1 , and an increased ratio of Bax: Bcl‐2 were detected by RT—PCR and Western blot analysis. Activation of caspase‐3 and caspase‐9 were not observed. This suggests that the apoptosis induced by MG132 in MG63 cells is caspase‐8 dependent, p27 and bcl‐2 family related.