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Actinobacillus actinomycetemcomitans adheres to human gingival fibroblasts and modifies cytoskeletal organization
Author(s) -
GutiérrezVenegas Gloria,
KawasakiCárdenas Perla,
Garcés Carla Portillo,
RománAlvárez Patricia,
BarajasTorres Carolina,
ContrerasMarmolejo Luis Arturo
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.03.028
Subject(s) - cytoskeleton , fibroblast , actinobacillus , intracellular , extracellular , fluorescence microscope , microbiology and biotechnology , actin , biology , chemistry , cell culture , cell , bacteria , fluorescence , biochemistry , physics , quantum mechanics , genetics
Adherence of Actinobacillus actinomycetemcomitans to human gingival fibroblast cells induces cytoskeletal reorganization. A. actinomycetemcomitans is considered a pathogenic bacteria involved in localized aggressive periodontitis. Studies with epithelial cells have shown an adherent capacity of bacteria that is increased under anaerobic conditions. For adherence to take place, there is a need for interaction between extracellular vesicles and bacterial fimbriae. However, molecular events associated with the adherence process are still unknown. The aim of this study was to investigate whether A. actinomycetemcomitans adherence to human gingival fibroblasts promotes cytoskeletal reorganization. Adherence was determined with light microscopy and scanning electron microscopy. For F‐actin visualization, cells were treated with fluorescein—isothiocyanate—phalloidin and samples were examined with epifluorescence optics. Fluorescent was recorded on Kodak T‐Max 400 film. We showed that A. actinomycetemcomitans adheres to human gingival fibroblast primary cultures, this property stimulating an increase in the intracellular calcium levels. In human gingival fibroblast primary cultures, we observed that maximal A. actinomycetemcomitans adherence took place 1.5 h after culture infection occurred and remained for 6 h. The adherence was associated with morphologic alterations and an increased in the intracellular calcium levels. These experiments suggest that A. actinomycetemcomitans adherence cause morphological alterations, induce actin stress fibers and recruitment of intracellular calcium levels.