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Reversal of multidrug resistance of vincristine‐resistant gastric adenocarcinoma cells through up‐regulation of DARPP‐32
Author(s) -
Hong Liu,
Wang Jin,
Han Ying,
Zhao Yunping,
Gao Juan,
Wang Jun,
Han Yu,
Zhang Xiaoyin,
Yan Li,
Zhou Xinmin,
Qiao Taidong,
Chen Zhen,
Fan Daiming
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.03.020
Subject(s) - multiple drug resistance , cancer research , transfection , vincristine , cancer , cisplatin , apoptosis , cancer cell , biology , cell culture , downregulation and upregulation , pharmacology , efflux , drug resistance , chemistry , gene , chemotherapy , biochemistry , cyclophosphamide , genetics
Here we investigated the roles of DARPP‐32 in multidrug resistance (MDR) of gastric cancer cells and the possible underlying mechanisms. We constructed the eukaryotic expression vector of DARPP‐32 and transfected it into human vincristine‐resistant gastric adenocarcinoma cell line SGC7901/VCR. Up‐regulation of DARPP‐32 could significantly enhance the sensitivity of SGC7901/VCR cells towards vincristine, adriamycin, 5‐fluorouracil and cisplatin, and could decrease the capacity of cells to efflux adriamycin. What's more, the results of subrenal capsule assay confirmed that DARPP‐32 might play a certain role in MDR of gastric cancer. DARPP‐32 could significantly down‐regulate the expression of P‐gp and zinc ribbon domain‐containing 1 (ZNRD1), but not alter the expression of multidrug resistance‐associated protein (MRP) or the glutathione S‐transferase (GST). DARPP‐32 could also significantly decrease the anti‐apoptotic activity of SGC7901/VCR cells. Further study of the biological functions of DARPP‐32 might be helpful for understanding the mechanisms of MDR in gastric cancer.