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Suppression of matrix metalloproteinase‐9 by 4‐methylumbelliferone
Author(s) -
Nakamura Rika,
Kuwabara Hiroko,
Yoneda Masahiko,
Yoshihara Shuichi,
Ishikawa Takashi,
Miura Tomisato,
Nozaka Hiroyuki,
Nanashima Naoki,
Sato Tatsusuke,
Nakamura Toshiya
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.03.016
Subject(s) - matrix metalloproteinase , chemistry , hyaluronidase , metalloproteinase , cell culture , biochemistry , enzyme , zymography , microbiology and biotechnology , matrix metalloproteinase 9 , matrix (chemical analysis) , biology , chromatography , genetics
OHK cells, a human lymphoma cell line, are known to produce large amounts of hyaluronan. We investigated the effect of 4‐methylumbelliferone, an inhibitor of hyaluronan synthesis, on the activity of matrix metalloproteinases in OHK cells. Matrix metalloproteinase‐9 was detected on gelatin zymography as the main metalloproteinase excreted into the medium of cultured OHK cells, and 4‐methylumbelliferone added to the medium decreased the activity of the enzyme in a dose‐dependent manner. Addition of Streptomyces hyaluronidase to the medium during cultivation did not decrease the enzyme activity. Reverse transcription‐polymerase chain reaction revealed that 4‐methylumbelliferone markedly decreased the level of mRNA for matrix metalloproteinase‐9 in cultured OHK cells. A similar decrease of the activity of matrix metalloproteinase‐9 by 4‐methylumbelliferone was also observed in cultured human breast and colon carcinoma cells. These results suggest that 4‐methylumbelliferone suppresses the expression of matrix metalloproteinase‐9 in cultured cancer cells.