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Influence of cytochrome c on apoptosis induced by Anagrapha ( Syngrapha ) falcifera multiple nuclear polyhedrosis virus (AfMNPV) in insect Spodoptera litura cells
Author(s) -
Liu Lijun,
Peng Jianxin,
Liu Kaiyu,
Yang Hong,
Li Yi,
Hong Huazhu
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.03.011
Subject(s) - cytochrome c , apoptosis , biology , mitochondrion , nuclear polyhedrosis virus , western blot , cytochrome , cytosol , spodoptera litura , microbiology and biotechnology , caspase 9 , caspase , apoptosome , virus , programmed cell death , virology , biochemistry , gene , lepidoptera genitalia , enzyme , botany
We investigated the influence of cytochrome c on apoptosis induced by Anagrapha ( Syngrapha ) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL‐1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent‐stained nuclei of AfMNPV‐infected SL‐1cells. Western blot analysis demonstrated that AfMNPV‐induced apoptosis in insect SL‐1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC‐DEVD‐AFC as substrate, the activity of caspase‐3 in AfMNPV‐induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase‐3 in apoptotic cells decreased in the presence of cyclosporin A (30 μM), indicating that activation of caspase‐3 in SfaMNPV‐induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (ΔΨm) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV‐induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis.