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Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture
Author(s) -
Schneider Nicole,
MouithysMickalad Ange,
Lejeune JeanPhilippe,
Duyckaerts Claire,
Sluse Francis,
DebyDupont Ginette,
Serteyn Didier
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.02.002
Subject(s) - oxygen , oxygen tension , chemistry , oxygenation , respiration , metabolism , cellular respiration , chondrocyte , carbohydrate metabolism , mole , biochemistry , medicine , endocrinology , biology , anatomy , mitochondrion , in vitro , organic chemistry
We investigated the oxygen (O 2 ) uptake of equine articular chondrocytes to assess their reactions to anoxia/re‐oxygenation. They were cultured under 5% or 21% gas phase O 2 and at glucose concentrations of 0, 1.0 or 4.5 g/L in the culture medium ( n = 3). Afterwards, the O 2 consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25 min. The glucose consumption and lactate release were measured at the end of the re‐oxygenation period. The chondrocytes showed a minimal O 2 consumption rate, which was hardly changed by anoxia. Independently from the O 2 tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5 g/L glucose ( n = 3). Lactate release was also independent from O 2 tension, but lower for cells at 4.5 g/L glucose ( n = 3). Our observations indicated that O 2 consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re‐oxygenation. But the chondrocytes metabolism was modified by an excess of O 2 and glucose.