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The apoptotic effect of sarsasapogenin from Anemarrhena asphodeloides on HepG2 human hepatoma cells
Author(s) -
Bao Wenna,
Pan Haifeng,
Lu Min,
Ni Yuan,
Zhang Rui,
Gong Xingguo
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.02.001
Subject(s) - apoptosis , dna fragmentation , flow cytometry , mtt assay , staining , fragmentation (computing) , cell cycle , viability assay , rhizome , microbiology and biotechnology , biology , chemistry , biochemistry , botany , programmed cell death , genetics , ecology
Sarsasapogenin, a kind of mainly effective components of Anemarrhena asphodeloides Bunge (Liliaceae) has the effects of being anti‐diabetes and improving memory. However, there are few reports focusing on its anti‐tumor effects. In this study, the sarsasapogenin was extracted from rhizomes of A. asphodeloides Bunge and applied to inhibit HepG2 human hepatoma cells. MTT assay showed that sarsasapogenin induced a distinct dose‐ and time‐dependent diminution of cell viability with IC 50 of 42.4 ± 1.0 μg/ml for 48 h. Furthermore, sarsasapogenin‐induced apoptosis of HepG2 cells was verified by Hoechst 33258 staining, electron microscopy, DNA fragmentation and PI staining. Flow cytometry analysis showed that sarsasapogenin‐induced cell apoptosis was through arrest of cell cycle in G 2 /M phase. Hence we proposed that sarsasapogenin could be used as an anti‐liver cancer drug for future studies.