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Epidermal growth factor (EGF) receptor endocytosis is accompanied by reorganization of microtubule system in HeLa cells
Author(s) -
Kharchenko Marianna V.,
Aksyonov Alexander A.,
Melikova Maria M.,
Kornilova Elena S.
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2007.01.020
Subject(s) - endocytosis , endosome , microbiology and biotechnology , microtubule , epidermal growth factor receptor , microtubule organizing center , epidermal growth factor , hela , receptor mediated endocytosis , tubulin , biology , chemistry , receptor , cell , centrosome , intracellular , biochemistry , cell cycle
Translocation of endosomes along microtubules (MTs) from the cell periphery toward the juxtranuclear region proximal to MTOC is well established. During this translocation the radial MT system is believed to retain its organization. Here we demonstrate that epidermal growth factor receptor (EGFR) endocytosis in HeLa cells is accompanied by dramatic remodeling of the MT system. Synchronized endocytosis was stimulated by warming the cells after EGF prebinding to EGFR on ice. Soon after that MTs were fully reestablished and EGFR was found in EE aligned along peripheral MTs. By the beginning of EE‐to‐LE sorting, the number of long MTs decreased and MTs appeared like an entangled meshwork of disorientated fragments and were partially depolymerized. Simultaneously, tubulin staining increased in juxtranuclear region, and at the time of LE‐Lys interaction, enlarged EGFR‐containing endosomes were localized there. Radial MTs were re‐established when EGF‐EGFR degradation started in lysosomes. In EGF absence, no alterations occurred upon MTs re‐establishment. We conclude that MT remodeling is endocytosis‐dependent.