Premium
Silencing invariant chain of DCs enhances Th1 response using small interfering RNA
Author(s) -
Ke Shan,
Chen XueHua,
Li Hao,
Li JianFang,
Gu QinLong,
Liu BingYa,
Zhu ZhengGang
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.12.004
Subject(s) - gene silencing , transfection , rna interference , small interfering rna , cytotoxic t cell , microbiology and biotechnology , chemistry , in vitro , rna , biology , gene , biochemistry
RNA interference (RNAi), which causes the degradation of any RNA in a sequence specific manner, is a posttranscriptional gene silencing mechanism. Targeting the invariant chain (Ii) in DCs has been used as an approach to enhance antitumor immunity. It is demonstrated in this article that transfection of H‐2 K DCs with siRNA specific for Ii gene can significantly knock down Ii. When exposed to TNF‐α, immature DCs transfected with Ii siRNA can differentiate into mature DCs without reducing viability or IL‐12p70 production. Ii siRNA‐treated H‐2 K DCs exhibited an increased allostimulatory capacity in a lymphocyte proliferation assay. Furthermore, Ii siRNA‐transfected H‐2 K DCs enhanced Th1 responses by increasing IFN‐γ and decreasing IL‐4 production, and much stronger cytotoxic activity was observed when DCs were co‐transfected with Ii siRNA and an endogenous tumor antigen in vitro . Our findings indicate that silencing the Ii gene in DCs with siRNA may offer a potential approach to enhancing antitumor immunotherapy.