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Retinal and olfactory bulb precursor cells show distinct responses to FGF‐2 and laminin
Author(s) -
Otaegi Gaizka,
Pablo Flora,
VicarioAbejón Carlos,
Rosa Enrique J.
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.11.031
Subject(s) - microbiology and biotechnology , olfactory bulb , neuroepithelial cell , embryonic stem cell , nestin , laminin , biology , fibroblast growth factor , olfactory ensheathing glia , extracellular matrix , neural stem cell , retinal , stem cell , immunology , neuroscience , central nervous system , biochemistry , receptor , gene
We analyzed whether the embryonic (E12.5–E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF‐2 plus heparin and laminin stimulated proliferation and the formation of aggregates composed, after two days, of 95.2% nestin‐positive cells. However, cells in these aggregates could only be passaged poorly, lost nestin expression and proliferative capacity, and differentiated into neurons. Under the same conditions, olfactory bulb precursor cells divided efficiently and could be expanded. These data suggest that, in addition to FGF‐2 and laminin, embryonic retinal neuroepithelial cells need additional extrinsic and/or intrinsic regulators to maintain cell proliferation and self‐renewal.