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Effect of nitric oxide on phagocytic activity of lipopolysaccharide‐induced macrophages: Possible role of exogenous l ‐arginine
Author(s) -
Tümer Cemil,
Bilgin Hakkı Murat,
Obay Basra Deniz,
Diken Hüda,
Atmaca Mukadder,
Kelle Mustafa
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.11.029
Subject(s) - lipopolysaccharide , nitric oxide , nitrite , arginine , macrophage , chemistry , intracellular , phagocytosis , pharmacology , biochemistry , endocrinology , immunology , medicine , in vitro , amino acid , organic chemistry , nitrate
Among the antimicrobial mechanisms associated with macrophages, NO produced by iNOS plays a major role in intracellular killing, but the relationship between NO and phagocytic activity after injection of inflammatory agents into the peritoneal cavity is not clear. The aim of the present study was to investigate the effect of nitric oxide (NO) on macrophage function after treatment with intraperitoneal lipopolysaccharide (LPS) and the role of exogenous l ‐arginine administration in this event. Six experimental groups and one control group, each consisting of seven Wistar rats were used: Group I: Control; Group II: LPS; Group III: LPS + l ‐arginine; Group IV: LPS + l ‐arginine + Aminoguanidine; Group V: LPS + Aminoguanidine; Group VI: l ‐arginine; Group VII: Aminoguanidine. Macrophage phagocytic activity and total plasma nitrite levels were increased in the LPS group. In the LPS + l ‐arginine group, both the phagocytic activity and total plasma nitrite levels showed large increases. Administration of aminoguanidine (AG), a specific iNOS inhibitor, abolished macrophage phagocytic activity and total plasma nitrite levels in the LPS and LPS + l ‐arginine groups. As a result, we showed that NO produced by macrophages has a role not only in intracellular killing, but also in phagocytic activity.