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Cytogenetic analysis of human bone marrow‐derived mesenchymal stem cells passaged in vitro
Author(s) -
Zhang ZhenXing,
Guan LiXue,
Zhang Kun,
Wang Shouxian,
Cao PeiCheng,
Wang YongHe,
Wang Zhe,
Dai LongJun
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.11.025
Subject(s) - mesenchymal stem cell , in vitro , bone marrow , biology , human bone , microbiology and biotechnology , immunology , genetics
Abstract This study concerns the cytogenetic stability of in vitro human bone marrow‐derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G‐banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.