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Antioxidant N ‐acetyl‐ l ‐cysteine inhibits erythropoietin‐induced differentiation of erythroid progenitors derived from mouse fetal liver
Author(s) -
Nagata Makiko,
Arimitsu Nagisa,
Ito Takahiro,
Sekimizu Kazuhisa
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.11.001
Subject(s) - gata1 , erythropoietin , microbiology and biotechnology , erythropoiesis , cellular differentiation , biology , progenitor cell , transcription factor , reactive oxygen species , chemistry , biochemistry , gene , stem cell , genetics , anemia , medicine
To determine the role of reactive oxygen species in erythroid differentiation, we investigated the effects of an antioxidant, N ‐acetyl‐ l ‐cysteine (NAC), on the differentiation of erythroid progenitors derived from mouse fetal liver. In response to erythropoietin (Epo), erythroid progenitors undergo differentiation in vitro and express erythroid‐specific genes such as β major ‐globin, Alas2, MafK, p45, Eklf , and Gata1 . Expression of these genes was decreased in the presence of NAC, whereas the expression of c‐myb , which is downregulated during erythroid differentiation, remained constant. Moreover, NAC treatment inhibited an increase in the number of cells expressing high levels of erythroid‐specific antigen TER119. Treatment with another antioxidant, pyrrolidine dithiocarbamate, also caused the attenuation of TER119 expression. These results suggest that reactive oxygen species are involved in Epo‐mediated erythroid differentiation.