Premium
Transformation by RAS oncogene decreases the width of substrate‐spread fibroblasts but not their length
Author(s) -
Kharitonova Margarita A.,
Kopnin Pavel B.,
Vasiliev Jury M.
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.10.008
Subject(s) - oncogene , transformation (genetics) , microbiology and biotechnology , polarity (international relations) , biology , confluence , cell culture , substrate (aquarium) , cell , cell cycle , genetics , gene , computer science , ecology , programming language
We compared morphometric parameters of the contours of cells in four pairs of non‐transformed mouse and rat lines and of the same lines transformed by oncogenes of the RAS family. As expected, the mean areas of all RAS ‐transformed lines were much smaller than those of their non‐transformed counterparts. At the same time the average length of cell projection did not regularly decrease after transformation. These results show that transformation induced by expression of RAS oncogene selectively affect only one component of spreading, namely transversal spreading and not longitudinal spreading; these changes result in an increase of antero‐posterior polarity of transformed fibroblasts.