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Neuroprotective effects of tetramethylpyrazine on hydrogen peroxide‐induced apoptosis in PC12 cells
Author(s) -
Cheng XinRui,
Zhang Li,
Hu JuanJuan,
Sun Lan,
Du GuanHua
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.10.001
Subject(s) - cytochrome c , apoptosis , tetramethylpyrazine , hydrogen peroxide , chemistry , cytosol , caspase , neuroprotection , microbiology and biotechnology , caspase 9 , caspase 3 , biochemistry , programmed cell death , biology , pharmacology , enzyme , medicine , alternative medicine , pathology
In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H 2 O 2 )‐induced apoptosis in PC12 cells. The apoptosis in H 2 O 2 ‐induced PC12 cells was accompanied by a decrease in Bcl‐2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase‐3. TMP not only suppressed the down‐regulation of Bcl‐2, up‐regulation of Bax and the release of mitochondrial cytochrome c to cytosol, but also attenuated caspase‐3 activation and eventually protected against H 2 O 2 ‐induced apoptosis. These results indicated that TMP blocked H 2 O 2 ‐induced apoptosis by the regulation of Bcl‐2 family members, suppression of cytochrome c release, and caspase cascade activation in PC12 cells.