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Ornithine decarboxylase overexpression enhances ERK and p38 phosphorylation and matrix metalloproteinase‐2 expression
Author(s) -
Nemoto Takahiro,
Kubota Shunichiro
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.09.019
Subject(s) - ornithine decarboxylase , ornithine decarboxylase antizyme , p38 mitogen activated protein kinases , mapk/erk pathway , microbiology and biotechnology , kinase , mitogen activated protein kinase , matrix metalloproteinase , phosphorylation , chemistry , biology , biochemistry , enzyme
Abstract In the present study, we investigated the relationship between ornithine decarboxylase, MAP kinase, and MMP‐2 expression in vitro . Overexpression of ornithine decarboxylase cDNA induced MMP‐2 expression both at mRNA and protein levels. Promoter analysis and gel shift assay showed that p53 and Ets‐1 were involved in MMP‐2 expression in ornithine decarboxylase overexpressing transfectants. Erk and p38 MAP kinase were significantly activated. Using specific inhibitors of MEK and p38, we clarified that MMP‐2 expression was induced via both Erk and p38 MAP kinase signaling pathways. This is the first report showing the existence of a causal relationship between ornithine decarboxylase expression, Erk and p38 MAP kinase activation, and MMP‐2 expression.