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G2 checkpoint‐dependent DNA repair and its response to catalase in Down syndrome and control lymphocyte cultures
Author(s) -
Pincheira Juana,
Romero Pablo,
Marcelain Katherine,
Salazar Lorena,
Torre Consuelo
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.09.018
Subject(s) - catalase , dna damage , g2 m dna damage checkpoint , mitosis , microbiology and biotechnology , intracellular , dna , dna repair , chemistry , lymphocyte , biology , cell cycle checkpoint , cell cycle , biochemistry , immunology , cell , enzyme
The amount of DNA lesions repaired in G2 and also G2 timing are controlled by the DNA damage‐dependent checkpoint. Down syndrome (DS) lymphocytes showed twice as much constitutive DNA damage in G2 than control ones, when recording it as chromosomal aberrations in metaphase, after caffeine‐induced checkpoint abrogation. During G2, DS lymphocytes repaired 1.5 times more DNA lesions than control ones. However the DS cells displayed a decreased threshold for checkpoint adaptation, as the spontaneous override of the G2 to mitosis transition block induced by the checkpoint took place in the DS cells when they had three times more DNA lesions than controls. Catalase addition to cultures scavenges hydrogen peroxide diffused from cells, resulting in subsequent intracellular depletion (Antunes and Cadenas, 2000). The intracellular H 2 O 2 level seemed to regulate the G2 checkpoint. Thus, in controls, H 2 O 2 depletion (induced by 3.2–50 μg/mL catalase) prevented its functioning: chromosomal damage increased while G2 shortened. Conversely, in the DS lymphocytes, 12.5 μg/mL catalase lengthened G2 and decreased chromosomal damage, in spite that the amount of DNA repaired in G2 was half of that repaired in the catalase‐free DS lymphocytes.