Premium
Effect of acrylamide on the cytoskeleton and apoptosis of bovine lens epithelial cells
Author(s) -
Arocena Miguel
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.07.008
Subject(s) - staurosporine , vimentin , acrylamide , colchicine , apoptosis , cytoskeleton , microbiology and biotechnology , actin , microtubule , intermediate filament , chemistry , biology , biophysics , cell , biochemistry , immunology , protein kinase c , genetics , immunohistochemistry , signal transduction , organic chemistry , polymer , copolymer
Acrylamide, a known disrupter of intermediate filaments, has been used to produce the collapse of vimentin filaments in bovine lens epithelial (BEL) cells, and its potential modulation of staurosporine‐induced apoptosis has been investigated. In BEL cells, short treatments with acrylamide caused the collapse of vimentin filaments and microtubules and the almost complete disappearance of stress fibers, with thick f‐actin bundles remaining in the cell periphery. Actin organization was less affected in cells pretreated with colchicine and in spreading cells, suggesting that extended microtubules and vimentin filaments are required for acrylamide to produce its maximal effects. Acrylamide alone slightly increased apoptosis compared to controls. However, simultaneous exposure to acrylamide and staurosporine for 8 h produced significantly less apoptosis than staurosporine alone, and preincubation with acrylamide followed by staurosporine markedly reduced apoptosis at 8 and 24 h of treatment. Acrylamide seems therefore to have a dual effect on BEL cell survival.