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Protein profiling and transcript expression levels of heat shock proteins in 17β‐estradiol‐treated human MCF‐7 breast cancer cells
Author(s) -
Lee SuUi,
Kim Bum Tae,
Min Yong Ki,
Kim Seong Hwan
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.07.005
Subject(s) - heat shock protein , biology , stathmin , proteomics , carcinogenesis , microbiology and biotechnology , protein disulfide isomerase , breast cancer , hsp90 , cancer research , gene , cancer , biochemistry , genetics , endoplasmic reticulum
Using proteomics, proteins regulated by 17β‐estradiol (E2) were identified in human MCF‐7 breast cancer cells; 26 proteins including heat shock proteins (Hsps) were differentially regulated by E2 in cells cultured in serum‐free condition. When the transcript levels of these proteins and another Hsps were measured by real‐time PCR, the transcripts encoding 6 proteins (Hsp56, Hsp90α, Hsp110, protein disulfide isomerase related protein, XTP3‐transactivated protein A and stathmin 1) were significantly up‐regulated and that encoding aminoacylase 1 was down‐regulated by E2 in cells cultured with or without serum. The protein profiling and transcript expression patterns of E2‐regulated proteins including Hsps in MCF‐7 cells suggested the involvement of these proteins in breast carcinogenesis. Proteins (or transcripts) whose expression pattern is altered by E2 might be potent targets for treating breast cancer patients.