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Direct association between caspase 3 and α5β1 integrin and its role during anoikis of rat fibroblasts
Author(s) -
Rajeswari J.,
Pande Gopal
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.06.022
Subject(s) - anoikis , cytosol , integrin , microbiology and biotechnology , chemistry , cell adhesion molecule , programmed cell death , cell adhesion , caspase 3 , apoptosis , cell , biology , enzyme , biochemistry
We have demonstrated a direct association between α5β1 integrin and caspase 3, both pro‐ and mature enzyme, in various sub‐cellular compartments of rat fibroblasts undergoing anoikis. Integrin associated caspase 3 showed high activity in the plasma membranes, whereas in the cytosol and microsomal fraction it showed little or no activity. Our results suggest a possible role for recycled un‐ligated α5β1 integrin molecules between cytosol and plasma membrane, in regulation of caspase‐3 activity and induction of cell death in adhesion‐deprived cells.