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Different transformation pathways of murine fibroblast NIH 3T3 cells by hepatitis C virus core and NS3 proteins
Author(s) -
Smirnova Irina S.,
Aksenov Nikolai D.,
Vonsky Maksim S.,
Isaguliants Maria G.
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.06.020
Subject(s) - ns3 , transactivation , 3t3 cells , hepatitis c virus , transfection , transformation (genetics) , virology , fibroblast , core protein , chemistry , virus , biology , microbiology and biotechnology , cell culture , gene expression , gene , biochemistry , genetics
The oncogenic potential of both Hepatitis C virus (HCV) core and HCV NS3 proteins has been demonstrated, but these proteins induce transformation of immortal murine fibroblasts NIH 3T3 via different pathways. As long‐term expression (50–100 passages) of HCV core triggers neoplastic transformation of NIH 3T3 through crisis of growth, HCV NS3 induces transformation shortly after transfection. We explain this distinction by different effects of core and NS3 on p53‐mediated transactivation: inhibition by NS3 and activation by core protein.