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Activation of bone marrow cells treated with Canova in vitro
Author(s) -
Abud Ana Paula Ressetti,
Cesar Beatriz,
Cavazzani Luiz Felipe Moscaleski,
Oliveira Carolina Camargo,
Gabardo Juarez,
Buchi Dorly de Freitas
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.06.011
Subject(s) - bone marrow , stromal cell , microbiology and biotechnology , flow cytometry , in vitro , progenitor cell , chemistry , mesenchymal stem cell , stem cell , biology , immunology , pathology , medicine , biochemistry
Canova is a Brazilian complex homeopathic medication produced from Aconitum, Thuya, Bryonia, Lachesis and Arsenicum . Previous studies demonstrated that Canova induces up‐regulation in numbers of leukocytes. The bone marrow microenvironment is composed of growth factors, stromal cells, extracellular matrix, and progenitor cells that differentiate into mature blood cells. As it is the major site of blood cell formation, we studied in vitro Canova effects on bone marrow cells of mice. Swiss mouse femurs were dissected, cleaned, and the marrow was flushed. The cells were plated, treated or not, incubated for different times and processed for light, scanning electron, and confocal microscopy, and also flow cytometry. The treatment did not modify the expression of the analyzed surface markers or cytokine production. All microscopy techniques showed that a monocytic lineage (CD11b + ) and stromal cells (adherent cells) were activated by treatment. Canova also increased cell clusters over adherent cells, suggesting proliferation areas.