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Gene delivery into primary cerebral cortical neurons by lentiviral vector
Author(s) -
Zhang Yuzhi,
Wang Hansen,
Pan Hong,
Bao Xinhua,
Li Meirong,
Jin Jing,
Wu Xiru
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.05.010
Subject(s) - viral vector , transduction (biophysics) , biology , green fluorescent protein , multiplicity of infection , in vitro , lentivirus , embryonic stem cell , gene delivery , virology , gene , microbiology and biotechnology , in vivo , cortical neurons , virus , genetic enhancement , recombinant dna , genetics , viral disease , biochemistry
Several studies have shown the ability of human immunodeficiency virus type 1 (HIV‐1)‐based lentiviral vectors to infect nondividing brain neurons. We are the first to show that primary embryonic cerebral cortical neurons can be efficiently transduced by an HIV‐1‐based lentiviral vector encoding enhanced green fluorescent protein (EGFP). We also describe the optimal conditions for the transduction of cerebral cortical neurons with lentiviral vectors, and the kinetic process of infection. The percentage of cells expressing EGFP is a function of the time in culture and virus dose. The highest percentage of EGFP‐expression achieved was 46.77% at 4 days in vitro (DIV) with a multiplicity of infection (m.o.i.) of 20. The results show that lentiviral vectors are not only good prospects for in vivo gene delivery, but are also good candidates for in vitro studies of the function of gene products in primary cerebral cortical neurons.