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Mechano‐chemical signaling in F9 cells
Author(s) -
Klemm Anna H.,
Suchodolski Klaudiusz,
Goldmann Wolfgang H.
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.05.007
Subject(s) - focal adhesion , microbiology and biotechnology , integrin , ptk2 , adhesion , protein kinase c , signal transduction , chemistry , cell adhesion , phosphorylation , cytoskeleton , intracellular , biophysics , biology , cell , biochemistry , mitogen activated protein kinase kinase , organic chemistry
We investigated the molecular mechanism by which cells recognize and respond to physical forces in their local environment. Using a model system, to study wild type mouse F9 embryonic carcinoma cells, we examined how these cells sense mechanical stresses and translate them into biochemical responses through their cell surface receptor integrin and via the focal adhesion complex (FAC). Based on studies that show that many signal transducing molecules are immobilized on the cytoskeleton at the site of integrin binding within the focal adhesion complex, we found a time‐dependent increase of focal adhesion kinase (pp125 FAK ) phosphorylation possibly due to protein kinase C (PKC) activation as well as protein kinase A (PKA) activity increase upon cell adhesion/spreading. These studies provide some insight into intracellular mechano‐chemical signaling.