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Algal toxin yessotoxin signalling pathways involve immunocyte mussel calcium channels
Author(s) -
Malagoli Davide,
Casarini Livio,
Ottaviani Enzo
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.05.003
Subject(s) - mytilus , verapamil , nitric oxide , mussel , toxin , intracellular , chemistry , calcium , marine toxin , biology , biochemistry , ecology , endocrinology , organic chemistry
A fragment of a putative L‐type Ca 2+ channel has been identified by molecular biology experiments in immunocytes from the mussel Mytilus galloprovincialis . Using the cell permeable and Ca 2+ ‐specific fluorochrome FURA 2‐AM, we have demonstrated that the algal toxin yessotoxin (YTX) is able to increase intracellular Ca 2+ concentration in M. galloprovincialis immunocytes. The YTX effect on Ca 2+ increase is inhibited by the L‐type Ca 2+ channel inhibitor, verapamil, which is cAMP‐ and cGMP‐dependent, but PKA‐ and nitric oxide‐independent. On the basis of these observations, a possible role for YTX as a potential disturber of mussel immune efficiency is suggested.