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Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana
Author(s) -
Wojtas M.,
Swiezewski S.,
Sarnowski T.J.,
Plochocka D.,
Chelstowska A.,
Tolmachova T.,
Swiezewska E.
Publication year - 2007
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.04.011
Subject(s) - rab , arabidopsis thaliana , gtpase , arabidopsis , biology , cloning (programming) , geranylgeranylation , microbiology and biotechnology , homology (biology) , gene , genetics , biochemistry , prenylation , enzyme , mutant , computer science , programming language
Intracellular vesicular trafficking is regulated by Rab proteins, small GTPases that require posttranslational geranylgeranylation for biological activity. This covalent modification is catalyzed by Rab geranylgeranyl transferase (RabGGTase) and proceeds only in the presence of accessory Rab Escort Protein (REP). In this communication, we report the cloning and characterization of REP gene of Arabidopsis thaliana . Highest expression of REP mRNA was detected in leaves and flowers in contrast to stems and roots. AtREP is recognized by anti‐rat REP1 serum. Interaction of AtREP with the protein substrate is presented, as well as a structural model obtained through homology modeling, based on the known structure of rat REP1.