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Effects of Gekko sulfated polysaccharide on the proliferation and differentiation of hepatic cancer cell line
Author(s) -
Wu XiongZhi,
Chen Dan,
Xie GuangRu
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.04.005
Subject(s) - sulfation , cell culture , apoptosis , cell growth , secretion , liver cancer , hepatocellular carcinoma , biology , hep g2 , microbiology and biotechnology , chemistry , cancer research , biochemistry , genetics
Gekko swinhonis Gūenther has been used as an anti‐cancer drug in traditional Chinese medicine. Gekko sulfated polysaccharide (Gepsin) was investigated for its activity in hepatocellular carcinoma. Hepatocarcinoma cell line (Bel‐7402) and liver cell line (L‐02) were exposed to Gepsin (100 μg/ml and 10 μg/ml). Gepsin did not suppress the proliferation and viability of normal liver L‐02 cells, but strongly inhibited the proliferation of hepatocarcinoma Bel‐7402 cells. Gepsin did not induce the apoptosis of Bel‐7402 cells, but blocked cells in G 2 /M phase. Treated with Gepsin, Bel‐7402 cells showed ultrastructural features of differentiation. AFP secretion decreased while ALB secretion increased markedly on Gepsin‐treated cells. The data show that Gepsin suppressed the proliferation and induced differentiation of hepatocarcinoma, but the toxicity to normal liver cells was negligible.