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Cellular isoform of the prion protein PrP c in human intestinal cell lines: Genetic polymorphism at codon 129, mRNA quantification and protein detection in lipid rafts
Author(s) -
Garmy Nicolas,
Guo XiaoJun,
Taïeb Nadira,
Tourrès Christian,
Tamalet Catherine,
Fantini Jacques,
Yahi Nouara
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2006.03.006
Subject(s) - prnp , lipid raft , gene isoform , biology , microbiology and biotechnology , messenger rna , cell culture , in vitro , caco 2 , gene , cell , gene expression , genetics , genotype
The cellular isoform of the normal prion protein PrP c , encoded by the PRNP gene, is expressed in human intestinal epithelial cells where it may represent a potential target for infectious prions. We have sequenced the PRNP gene in Caco‐2 and HT‐29 parental and clonal cell lines, and found that these cells have a distinct polymorphism at codon 129. HT‐29 cells are homozygous Met/Met, whereas Caco‐2 cells are heterozygous Met/Val. The 129Val variant was also detected in Caco‐2 mRNAs. Real‐time PCR quantifications revealed that PrP c mRNAs were more expressed in HT‐29 cells than in Caco‐2 cells. These data were confirmed by studying the expression of PrP c in plasma membranes and lipid rafts prepared from these cells. Overall, these results may be important in view of using human intestinal cell lines Caco‐2 and HT‐29 as cellular in vitro models to study the initial steps of prion propagation after oral inoculation.