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Hydrogen peroxide‐induced apoptosis in human gastric carcinoma MGC803 cells
Author(s) -
Mao Yubin,
Song Gang,
Cai Qiufeng,
Liu Min,
Luo Haohong,
Shi Mingxin,
Ouyang Gaoliang,
Bao Shideng
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.12.008
Subject(s) - apoptosis , hydrogen peroxide , oxidative stress , chemistry , cancer cell , programmed cell death , reactive oxygen species , downregulation and upregulation , mitochondrion , viability assay , microbiology and biotechnology , cancer , cancer research , biology , biochemistry , gene , genetics
Hydrogen peroxide (H 2 O 2 ), a representative ROS, has been used to study the apoptosis of cancer cells to oxidative stress. In this study, we exploited the cellular and molecular mechanisms involved in H 2 O 2 ‐induced apoptosis in human gastric carcinoma MGC803 cells. Exposure of cells to H 2 O 2 might cause significant viability loss and the increase in apoptotic rate. Treatment with 0.4 mmol/L H 2 O 2 up‐regulated Bax but down‐regulated Bcl‐2 in a time‐dependent manner, while Bcl‐xL expression remained unchanged. Our results also showed that the levels of Fas and Fas‐L were increased, the pro‐caspase‐3 and pro‐caspase‐9 were down‐regulated in H 2 O 2 ‐treated MGC803 cells. Under H 2 O 2 stress, we found that the protein p53 also participated in MGC803 cells apoptosis. Taken together, the present study indicated that Fas‐mediated cell surface death receptor pathway and mitochondria‐mediated pathway may participate in regulating the MGC803 cells apoptosis under oxidative stress.