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Induction of transforming growth factor‐beta 1 on dentine pulp cells in different culture patterns
Author(s) -
Nie Xin,
Tian Weidong,
Zhang Yongjie,
Chen Xizhe,
Dong Rui,
Jiang Ming,
Chen Famin,
Jin Yan
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.12.001
Subject(s) - odontoblast , pulp (tooth) , chemistry , microbiology and biotechnology , dentinal tubule , dental pulp stem cells , dentistry , transforming growth factor , transforming growth factor beta , dentin , biology , stem cell , medicine
Recent studies have documented that TGF‐β1 takes part in dental pulp tissue repair. Moreover, dental pulp cells have the potential to differentiate into odontoblast‐like cells and produce reparative dentine in this process. However, the molecular mechanisms and potential interactions between TGF‐β1 and dental pulp cells are not clear due to the complexity of the pulp/dentine microenvironment. In this study, we investigated the induction of TGF‐β1 on the dental pulp cells in cell culture, tissue culture and three‐dimensional culture patterns. These results demonstrated that TGF‐β1 significantly increased the proliferation of cells and activity of ALPase. Dental pulp cells cultured in the presence of TGF‐β1 formed mineralization nodules. In the organ culture, dental pulp cells treated with TGF‐β1 differentiated into odontoblast‐like cells and formed a pulp‐dentinal complex; and TGF‐β1 significantly induced synthesis of dentine relative proteins DSPP, DMP‐1. The dental pulp cells share some characteristics of the odontoblast, such as a parallel arrangement with columnar form and a unilateral cell process. Together, these data indicate that TGF‐β1 can make dental pulp cells differentiated into odontoblast‐like cells and form the pulp‐dentinal complex. Moreover, these results suggest that TGF‐β1 is an important regulatory factor in odontoblast differentiation during tooth development and pulp repair.