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Role of protein conformation and aggregation in pumping water in and out of a cell
Author(s) -
Cameron I.L.,
Kanal K.M.,
Fullerton G.D.
Publication year - 2006
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.09.010
Subject(s) - chemistry , dextran , shaker , biophysics , urea , chromatography , osmotic shock , salt (chemistry) , crystallography , biochemistry , organic chemistry , biology , physics , quantum mechanics , gene , vibration
Dialysis cassettes containing BSA solutions were used to simulate passive in vivo conditions to assess the effect of protein conformation and aggregation on cell water content. The cassettes were suspended in dextran solutions to provide a range of fixed osmotic stress values simulating blood plasma. The system was placed on a shaker for 24 h to attain equilibrium. Four manipulation methods; pH, cosolute salt concentration, e.g. NaCl, temperature annealing and urea concentration denaturant were varied to produce well‐known manipulations of BSA conformation. It was observed that the cell water content varied from +14% to about −13% with changes in protein conformation and aggregation. The findings demonstrate that a change in protein conformation and aggregation, pumps water in and out of a cell to maintain equilibrium % water content matching the protein conformational hydration parameter. This concept supplements existing theories on cell volume regulation.