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Generation of embryoid bodies from mouse embryonic stem cells cultured on STO feeder cells
Author(s) -
Zhou QingJun,
Shao JianZhong,
Xiang LiXin,
Hu RuoZhen,
Lu YongLiang,
Yao Hang,
Dai LiCheng
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.05.007
Subject(s) - embryoid body , embryonic stem cell , endoderm , microbiology and biotechnology , germ layer , embryo , biology , stem cell , petri dish , chemistry , anatomy , adult stem cell , genetics , induced pluripotent stem cell , gene
Embryoid bodies, which are similar to post‐implantation egg‐cylinder stage embryos, provide a model for the study of embryo development and stem cell differentiation. We describe here a novel method for generating embryoid bodies from murine embryonic stem (ES) cells cultured on the STO feeder layer. The ES cells grew into compact aggregates in the first 3 days of coculture, then became simple embryoid bodies (EBs) possessing primitive endoderm on the outer layer. They finally turned into cystic embryoid bodies after being transferred to Petri dishes for 1–3 days. Evaluation of the EBs in terms of morphology and differentiating potential indicates that they were typical in structure and could generate cells derived from the three germ layers. The results show that embryoid bodies can form not only in suspension culture but also directly from ES cells cultured on the STO feeder layer.