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Effects of insulin‐like growth factor‐1 on okadaic acid‐induced apoptosis in SH‐SY5Y cells
Author(s) -
Xing Changhong,
Peng Ying,
Chang Rui,
Yin Yanling,
Xie Zuoping
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.04.012
Subject(s) - propidium iodide , apoptosis , annexin , okadaic acid , cytotoxicity , mtt assay , viability assay , microbiology and biotechnology , flow cytometry , insulin like growth factor , biology , chemistry , endocrinology , medicine , growth factor , programmed cell death , biochemistry , in vitro , phosphatase , phosphorylation , receptor
The effects of insulin‐like growth factor‐1 (IGF‐1) on the cytotoxicity and apoptosis induced by okadaic acid (OA) in SH‐SY5Y cells were investigated. Cell viability was measured using the MTT (3‐(4,5‐dimethylthiazolyl‐2)‐2,‐5‐diphenyltetrazolium bromide) assay. Early and late apoptosis/necrosis were analyzed by flow cytometry using Annexin V and propidium iodide (PI) double‐staining. Caspase‐3 activation was detected by Western blot analysis. Preincubation with IGF‐1 for 24 h prevented cytotoxicity induced by 40 nM OA given for 24 h, and the MTT value significantly increased. Incubation with 20 nM OA for 24 h caused a marked increase in the percentage of early apoptotic and late apoptotic/necrotic cells, which was not dependent on the activation of caspase‐3. OA‐induced apoptosis was significantly decreased by pretreatment with 10 ng/ml of IGF‐1 for 24 h. The results supported the hypothesis that IGF‐1 may be useful in the treatment of Alzheimer's disease.