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Prediction of VEGF mRNA antisense oligodeoxynucleotides by RNA structure software and their effects on HL60 and K562 cells
Author(s) -
Fei Jia,
Zhang Yuan
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.04.009
Subject(s) - trypan blue , hl60 , k562 cells , messenger rna , microbiology and biotechnology , rna , flow cytometry , cell growth , antisense rna , biology , sense (electronics) , viability assay , apoptosis , chemistry , biochemistry , gene
Seven antisense oligodeoxynucleotides were selected using RNA structure 3.7 software and the principle of low overall Δ G (free energy). Their effects on cell growth, VEGF protein expression and apoptosis in HL60 and K562 leukemic cells were examined, and cell numbers and viability were assessed using trypan blue dye exclusion, MTT, ELISA and flow cytometry. The results showed that six of the seven antisense sequences inhibited the cell growth and down‐regulated VEGF protein expression significantly. Endogenous VEGF plays an important role in the proliferation of HL60 and K562 leukemic cells. RNA structure software provides a rapid and efficient way of identifying effective antisense oligodeoxynucleotides.