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Evidence that erythrocytes are highly susceptible to exercise oxidative stress: FT‐IR spectrometric studies at the molecular level
Author(s) -
Petibois Cyril,
Déléris Gérard
Publication year - 2005
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1016/j.cellbi.2005.04.007
Subject(s) - chemistry , oxidative stress , hemoglobin , protein carbonylation , oxidative phosphorylation , biochemistry , denaturation (fissile materials) , lipid peroxidation , nuclear chemistry
We tested the hypothesis that usual exercise oxidative stress strongly affects erythrocytes viability. A 120‐min physical exercise with progressive intensity was used as a model of oxidative stress. FT‐IR spectrometry was used to determine structural changes in erythrocyte contents (phospholipids, proteins, lactate, and glucose) from blood samples taken every 20 min. Carbonyl formation from amino acid residues ( P = 0.03) and hemoglobin unfolding ( P = 0.01) could be identified as main protein denaturation markers during oxidative stress. Higher unsaturation level ( P = 0.001) in phospholipids fatty acyl chains were also observed while VO 2 increased ( P < 0.05). The increase in lactacidosis affected primarily hemoglobin unfolding ( P = 0.02). Finally, two distinct cellular events occurred during oxidative stress: 1—phospholipids peroxidation correlated to VO 2 , but lactacidosis and hemoconcentration remained secondary factors; 2—hemoglobin denaturation was mainly observed through unfolding and carbonylation, and lactacidosis and hemoconcentration were important contributing factors.